FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758102

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758102
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences549260
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15350.039.0108261
GTACGGT400.002640965434.3771173
TCAACGC17400.034.3708574
ATCAACG17750.033.699263
CAACGCA17750.033.6900565
AACGCAG18450.032.039296
GTGGTAT3050.031.5938421
TATCAAC19500.030.5181262
GGTATCA7000.029.9899711
ACGCAGA19750.029.9303767
CGCAGAG20400.028.9767138
CAGAGTA19800.028.63977410
AGAGTAC20250.028.00333611
CCGTAAG500.00767681627.6401238
GCAGAGT22000.027.0255329
TGGTATC3550.026.1626532
TAATCGT1208.382267E-825.91261338
TAGTTCG1509.0403773E-1025.6062757
AGTACGG18400.024.65511113
GAGTACG18850.024.43117112
Produced by FastQC (version 0.11.5)