FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758104

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758104
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences822167
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11330.1378065526833356No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24600.038.7292671
GGTATCA10750.033.7930221
TATCAAC28750.033.1165962
ATCAACG29850.031.5347443
TCAACGC29900.031.3618054
AACGCAG30600.030.869946
CGCTTAT450.00480762130.420712
CAACGCA30800.030.336125
ACGCAGA32650.028.8268817
AGAGTAC33050.027.96020911
CAGAGTA33050.027.85665310
CGCAGAG34550.027.241618
GCAGAGT35150.026.8739749
TAACCGT808.586774E-526.2861353
CGCGGTA809.1397094E-526.04604543
GTATAAG2250.025.8938141
TAACGGC809.609944E-525.85457636
CTAACCG1001.3899502E-524.51845452
TTAACGG851.4559855E-424.32031235
AGTACGG27700.023.84510413
Produced by FastQC (version 0.11.5)