FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758106

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758106
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences647400
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11900.183812171763979No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTAG350.001290673339.77485746
GTATCAA15950.038.7017821
CAATCGT400.002217314735.6253865
CGATTGG800.003097922333.27210670
CGTACTA450.00399463531.59336964
TCGTACG450.00406728631.47726262
GTCTAGC1208.367351E-1131.4362411
TATCAAC20050.030.768662
ATCAACG20000.030.4816423
AACGCAG19800.030.429486
CAACGCA19950.030.3722825
TCAACGC20200.030.165864
GGTATCA6900.029.8209421
ACGCAGA21600.027.893697
AGAGTAC21350.027.25825711
CAGAGTA21950.026.82507510
CGCAGAG22600.026.8109288
GCAGAGT24000.025.2469589
GTGGTAT3850.024.941151
GTATACC700.00138053724.4957731
Produced by FastQC (version 0.11.5)