FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758108

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758108
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2473
Sequences flagged as poor quality0
Sequence length21-76
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG240.9704811969268096No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.2426202992317024No Hit
CTCCAGGGAGCGCCATCATCAACGCAAACCGGCAATGTAGTCGGCAACAG30.1213101496158512No Hit
GTGCAAAACACCGCCTTGCAACACCTGCAAACCGGCCATTGGGTCAACCT30.1213101496158512No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.1213101496158512No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.1213101496158512No Hit
GAAGGATTGCTCGAAATAAAGTCCCATAGTGGAGCACGGGTTGCTGCATT30.1213101496158512No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.1213101496158512No Hit
GTATTCGTTCCAGCTGCGCGGCGACTGATGCAGCAGCAGCACGGGCGGTG30.1213101496158512No Hit
TCCATGCAGGCCGGCCATCTGCCCGAAGGATTGGCGCGGAGCCTCCACGA30.1213101496158512No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCAG200.00684784551.107146
ACGCAGA200.00684784551.107147
GTATCAA200.00684784551.107141
TCAACGC200.00684784551.107144
CAGAGTA200.00684784551.1071410
AGTACGG200.00684784551.1071413
AGAGTAC200.00684784551.1071411
GAGTACG200.00684784551.1071412
GCAGAGT200.00684784551.107149
TATCAAC200.00684784551.107142
GTACGGG200.00684784551.1071414
Produced by FastQC (version 0.11.5)