FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758112

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758112
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences799511
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11670.14596422062986No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTA350.001396523239.1433915
GTATCAA22650.035.8696481
TGGTATC5200.034.2547532
GTGGTAT5450.033.3369871
GGTATCA10600.031.046481
TACGATT450.004756761730.48681427
TCAACGC27600.029.158894
ATCAACG27750.029.1283283
TATCAAC28050.028.942522
CAACGCA27850.028.7741745
AACGCAG28750.028.111656
AGAGTAC29800.027.0062211
ACGCAGA30000.026.9403347
CAGAGTA30550.026.2311210
CGCAGAG31600.025.6846398
CAATCGT700.001219087225.02497555
GCAGAGT32350.024.7715829
GAGTACT10350.022.49990812
TACCGGA800.003021153221.4092222
AGTACTT11250.020.39550613
Produced by FastQC (version 0.11.5)