FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758113

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758113
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences291005
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATAGA400.00965970648.28774370
GTATCAA12650.042.2536161
GTGGTAT1550.041.732721
AAAATCG350.001300334539.70706642
GGTATCA5400.035.9365041
TATCAAC15850.034.1525082
GTATGTA400.002767532634.0451131
AACGCAG17050.032.1481676
CAACGCA17100.032.054175
TCAACGC17150.031.7622014
ATCAACG17450.031.4112473
ACGCAGA18700.029.306537
CGCAGAG19100.028.692788
AGAGTAC19350.028.14615811
AAGTATG755.69369E-527.91243644
CAGAGTA19550.027.85821710
GCAGAGT19850.027.608679
TACACGC500.00824242827.2360885
TGGTATC2550.026.7020452
ACGGGGC1751.0913936E-1125.29065516
Produced by FastQC (version 0.11.5)