FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758115

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758115
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1022935
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG704.2564352E-1044.53743440
CGTATGC651.08939275E-842.67209641
GTATCAA33100.039.7350461
GGTATCA14100.036.9228321
ACGCTAG400.002623754834.4257933
TATCAAC38350.034.2954372
TCAACGC39250.033.601244
ATCAACG39050.033.5945973
CAACGCA40150.032.9349635
AACGCAG40150.032.849646
ACGCAGA44100.029.9073247
CGAGACG3100.029.85029623
TCTCGTA1052.2870154E-829.62864138
AGAGTAC43850.029.60474811
ACGCTCA3500.029.40956527
CGCAGAG45550.028.8800728
CAGAGTA45800.028.64622910
CGCTCAT3250.028.52001828
GTGGTAT5350.028.1688671
ACGAGAC3300.028.03707322
Produced by FastQC (version 0.11.5)