FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758116

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758116
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1022935
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25750.2517266492983425No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA35200.038.7229041
GGTATCA15200.036.27521
TATCAAC40300.033.627842
ATCAACG41800.032.1660463
TCAACGC42000.031.9251754
AACGCAG42450.031.6673226
CAACGCA42600.031.395235
AATCGGA450.004807710630.42128417
ACGCAGA45850.029.3190387
AGAGTAC44650.029.03449411
CGCAGAG46250.028.6956778
CAGAGTA45900.028.39283410
GCAGAGT48850.027.0983529
TGGTATC7350.025.6085032
GTGGTAT7200.024.7342281
GAGTACG35350.023.22312
AGTACGG35600.022.96383313
TAGACCG750.002094699322.8036965
GAGTACT21300.022.48251712
AGTACTT21250.022.37444913
Produced by FastQC (version 0.11.5)