FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758119

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758119
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1125872
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15250.040.4429931
GTATCAA29750.034.897661
ATCAACG35950.028.9744593
TCAACGC36000.028.8390394
CAACGCA37200.027.9087475
AACGCAG37450.027.5406766
TATCAAC38800.026.757872
TCGTATG1057.466642E-726.3719740
GTAGTTT5450.025.97704170
ACGCAGA40400.025.6099287
AGAGTAC40300.025.16340411
CGCAGAG41600.024.7888248
CAGAGTA41400.024.41206210
GCGTAGA1851.4656291E-524.34948770
GTGGTAT5800.024.2212471
CGTATGC1151.6319045E-624.10904741
TGGTATC6250.023.0255452
GCAGAGT45100.022.9410449
AGTACGG34550.022.11160713
GAGTACG34500.022.04533212
Produced by FastQC (version 0.11.5)