FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758120

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758120
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1125872
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18470.16405062031918372No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29450.036.429611
GGTATCA13250.031.818871
TCAACGC36100.029.3026164
ATCAACG36400.029.2531073
AACGCAG36900.029.0384256
GTGGTAT6650.028.8643551
CAACGCA37450.028.429145
TATCAAC38100.028.317372
ACGCAGA39750.026.9564277
TAACGCT659.057474E-426.3336944
AGAGTAC40450.026.15140711
TGGTATC7750.026.07462
CAGAGTA41250.025.89320410
CGCAGAG41750.025.5831038
GCAGAGT44800.023.9942259
GTATAAC1151.796383E-623.844471
TATTCGA750.00187054323.25211549
CGAACTA5250.022.88045529
AGTACGG36500.022.32231513
CGGACTA1453.9669794E-722.06483764
Produced by FastQC (version 0.11.5)