FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758122

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758122
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences977364
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14470.14805128897728992No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGATT650.00114433640.7586270
GTATCAA24350.036.660661
GTGGTAT5350.031.4462011
GGTATCA11950.031.3173121
TCAACGC29050.030.6963164
ATCCGTT802.4286073E-630.68830958
AACGCAG28850.030.6713546
TATCAAC29000.030.6435182
CAACGCA29300.030.200295
ATCAACG29700.029.9105663
ACGCAGA31750.027.97797
AGAGTAC32000.026.9018911
CAGAGTA32450.026.5288310
CGCAGAG33700.026.3590018
GCAGAGT35050.025.2458959
TGGTATC6950.024.1906832
TAATCGT1854.7293724E-1022.41426838
AGTACGG30850.021.90131213
GAGTACG30950.021.8305512
ACGGGTA2252.7830538E-1019.81719216
Produced by FastQC (version 0.11.5)