FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758124

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758124
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1055113
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13190.12501030695290458No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTACGT754.539495E-543.83477470
GTATCAA27950.034.102341
GGTATCA13850.031.1919121
TAACGCT703.2821157E-530.27171560
CGAATTA704.0526684E-529.35069715
AACGCAG33400.028.39876
TCAACGC33650.028.0859534
CAACGCA34000.027.9982595
ATCAACG33950.027.8417283
TATCAAC35200.027.1577852
ATTACGT906.551003E-627.01585245
ACGCAGA35350.026.8321517
GTGGTAT6250.026.3319151
AGAGTAC35400.026.21263311
AACGCTA958.665556E-626.05999261
CAGAGTA36100.025.70557210
CGCAGAG37300.025.2457948
GCAGAGT37900.024.8461259
TACGTTA1001.4621066E-524.35993847
AATTACG1001.4940448E-524.29207844
Produced by FastQC (version 0.11.5)