FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005758125

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758125
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences975659
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT19850.20345223074865298No Hit
GTTAGGACCCGGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC17310.17741854479895125No Hit
GTATAATAATTGGCGAGGCACCTTCGGCCAAGGGACACGACTGGACATTAAACGAACTGTGGCTGCACCAT14160.14513267442825822No Hit
TTATTATACTGCTGACAGTAATAAACTGCAGAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTC13640.13980294344642955No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13510.13847051070097238No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12670.12986094526878755No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT12250.12555616255269514No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11910.12207133844919177No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT11860.12155886431632362No Hit
CATCTATGGTGCGTCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA11570.11858651434568841No Hit
CTCCTGTAGGGCCAGTCAGAGTGTGCACAGCTATTTAGTCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT11250.11530667989533229No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11130.11407674197644875No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11040.11315428853728608No Hit
TCTATGGTGCGTCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT10580.10843952651489917No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGCCTC10430.10690210411629472No Hit
GTGTAAGGATCACCTGGTTTTTGGAGGCGTCCTTGGTGATGTTGAGTCTG10420.10679960928972111No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGAC10300.10556967137083757No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTCTGCAGTTTATTACTGTCAGCAGTATAATAATTGG10230.10485220758482217No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10090.10341728001279135No Hit
TCCTTACACTGTCCAACGTGGACCCTGGGGACACAGCCATATATTTCTGTGCACACGCTCCATACGTGGATACAA10050.10300730070649684No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)