FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005758127

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758127
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2853294
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG71660.2511483219044375No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT69550.24375336015146No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC52140.18273616388637132No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC50390.17660290176897298No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC49050.17190657534765083No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG47860.16773595710781994No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG47500.16647425747224087No Hit
GTCTCTGGTGGCTCCATCAGAAGTGATGATTACTACTGGAGTTGGATCCG47310.1658083604423519No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG45620.15988538159755006No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC43260.15161423954208714No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC43210.15143900348159006No Hit
GTCTCCATCCTCCCTGTCTGCATCTGCAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGGCATTGCTAA40460.14180102015424978No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT39310.13777059076281659No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT38010.1332144531898921No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT37160.13023544016144148No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT36180.1268008133756984No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG34650.12143858992448726No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT34010.11919556835012445No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT33810.11849462410813608No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC32530.11400858095941044No Hit
CAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTACTGATGGTGAAAGTAAAATCTGTCCCAGATCCACGTCCA32120.11257164526333424No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT32110.11253659805123481No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG31800.11145013447615283No Hit
GTAATATGTTGCAATATCTTCAGGCTGCAGGCTACTGATGGTGAAAGTAAAATCTGTCCCAGATCCACGTCCA31380.10997815156797723No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT31350.10987300993167896No Hit
AGTCAGGGCATTGCTAACGGTCTAAATTGGTATCAGCAGAAACCAGGGAA30740.10773512999361438No Hit
GGCTACTGATGGTGAAAGTAAAATCTGTCCCAGATCCACGTCCACTGAAC30250.10601781660074287No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG29690.1040551727231754No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG29650.1039149838747777No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC29610.10377479502638003No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT29610.10377479502638003No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATTCACTTTGGTCC29180.10226776490610502No Hit
GTGCAGCCACAGTTCGTTTGATATTCACTTTGGTCCCAGGGCCGAATCGA29120.1020574816335085No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC29070.1018822455730114No Hit
TCAGTAGCCTGCAGCCTGAAGATATTGCAACATATTACTGTCAACAATAT28840.10107615969472478No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG28740.10072568757373059No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC28690.10055045151323347No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)