FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005758138

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758138
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6075
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG731.2016460905349795No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC120.19753086419753088No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT110.18106995884773663No Hit
GTGTACTACTGTCAACATTATGGTACCTCACCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA100.1646090534979424No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT80.13168724279835392No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT80.13168724279835392No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC80.13168724279835392No Hit
GTAGTACACTGCACAATCTTCAGGCTCCAGTCTGGTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA70.11522633744855967No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)