FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758145

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758145
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2989585
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAAGTGTGTTGACAGTAGTAAGTTCCAAAATCTTCAGGTTGCAGACTGGTGATGGTGAGAGTGAAATCTGTC72400.24217408101793395No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT71870.2404012597066148No Hit
CACTTACACTACCCCGTTCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT70190.2347817506443202No Hit
GGGTAGTGTAAGTGTGTTGACAGTAGTAAGTTCCAAAATCTTCAGGTTGCAGACTGGTGATGGTGAGAGTGAAAT65470.21899360613596872No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA54960.18383822503792333No Hit
GTAATACACGGCCGTGTCCGCGGCAGAAACAGAACTCAGATTCAGGGAGA51950.17376993796797885No Hit
ACACTACCCCGTTCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA48800.16323335847617645No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG48580.1624974703846855No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG44990.1504891147099012No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG44620.14925148473784824No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT42950.14366542513425778No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC42070.14072187276829393No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41200.13781176986103422No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG40200.13446682399062077No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG40190.13443337453191662No Hit
GTCTCTGGTGGCTCCATCAGCAGTGGTGTTTACTTCTGGAGCTGGATCCG40000.13379783481653806No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG38260.12797762900201867No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC38110.12747588712145666No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG37640.12590376256236235No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT37540.125569267975321No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGCGTCCAATCAAGGTTCAGTGGCAGTGGGTCTGGGACAGATTTCA37060.12396369395752252No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAT36340.12155533293082485No Hit
GTCGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCATCTCTTTACATTGGTATCAGCACAAA36300.12142153509600831No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT36260.12128773726119177No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC36080.12068564700451734No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT34820.11647101520779639No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC33440.11185498990662585No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT33370.11162084369569689No Hit
GTGTATTACTGTGCGAGAGTTGACTACCGTAACTACGTTTTTGACCTCTGGGGCCAGGGAACCCTAGTCACCGT32690.10934628050381576No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT32010.1070717173119346No Hit
GTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA31510.10539924437672787No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT30810.10305778226743845No Hit
CACCATCACCAGTCTGCAACCTGAAGATTTTGGAACTTACTACTGTCAAC30090.10064942124074078No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA54900.040.6639331
TCGTATG2750.036.49618540
TACTGAA21700.036.07121770
TATCAAC61650.035.988492
ATCAACG65500.033.8737073
TCAACGC65250.033.8464664
CAACGCA65650.033.6926425
AACGCAG66700.033.162256
TATCTCG3100.032.30876536
GTGGTAT8000.032.248411
ACGCAGA73100.030.347897
CGCAGAG75100.029.6312898
TGTGTCG24150.029.39037770
AGAGTAC75450.029.21885311
TATACAC5550.029.1305833
CAGAGTA79450.027.87859310
GCAGAGT80350.027.7808179
CGTATGC3800.026.4237241
ACGAGAC4850.026.24288222
CATAGGT14950.025.77043
Produced by FastQC (version 0.11.5)