FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758146

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758146
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2989585
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT72500.24250857560497527No Hit
GTGTAAGTGTGTTGACAGTAGTAAGTTCCAAAATCTTCAGGTTGCAGACTGGTGATGGTGAGAGTGAAATCTGTC71970.24073575429365612No Hit
CACTTACACTACCCCGTTCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT63490.21237061331255006No Hit
GGGTAGTGTAAGTGTGTTGACAGTAGTAAGTTCCAAAATCTTCAGGTTGCAGACTGGTGATGGTGAGAGTGAAAT62410.20875807177250355No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG57690.19296992726415205No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA56080.18758456441278637No Hit
ACACTACCCCGTTCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA48060.1607580985320705No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT45630.15262988006696582No Hit
GTAATACACGGCCGTGTCCGCGGCAGAAACAGAACTCAGATTCAGGGAGA44850.1500208222880433No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44530.14895043960951102No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG43460.14537134752816863No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG41430.1385811074112293No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC41340.1382800622828921No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG39470.13202501350521895No Hit
GTCTCTGGTGGCTCCATCAGCAGTGGTGTTTACTTCTGGAGCTGGATCCG37870.12667310011255745No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG37620.12583686364495406No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGCGTCCAATCAAGGTTCAGTGGCAGTGGGTCTGGGACAGATTTCA37150.12426473908585974No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAT36850.12326125532473571No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT36480.12202362535268273No Hit
GTCGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCATCTCTTTACATTGGTATCAGCACAAA36250.12125428780248763No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC36230.12118738888507938No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35670.11931421919764783No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC34740.11620341953816334No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC34610.11576857657500958No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT34230.11449749714425247No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG33330.11148704586088035No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT32460.10857694295362066No Hit
GTGTATTACTGTGCGAGAGTTGACTACCGTAACTACGTTTTTGACCTCTGGGGCCAGGGAACCCTAGTCACCGT32060.10723896460545528No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT31830.10646962705526018No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT31750.10620203138562709No Hit
CACCATCACCAGTCTGCAACCTGAAGATTTTGGAACTTACTACTGTCAAC30940.1034926252305922No Hit
GTCTGGGACAGATTTCACTCTCACCATCACCAGTCTGCAACCTGAAGATTTTGGAACTTACTACTGTCAACACA30630.10245569201076403No Hit
GTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA29940.10014767936017877No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA58850.039.543841
GTGGTAT9700.038.6270451
TATCAAC65650.035.1631432
TACTGAA20800.034.2530270
ATCAACG69700.033.2024043
TCAACGC69900.033.003644
CAACGCA70300.032.913525
AACGCAG72150.032.069586
ACGCAGA77350.029.7361157
AGAGTAC78650.028.8517711
CGCAGAG79700.028.8167368
GCAGAGT84150.027.2928569
CAGAGTA83400.027.24969710
CTGCAGT27100.026.79090570
GTGAGCG13700.025.75465270
ATAGGTG18600.025.6549764
TAGGTGG20000.024.717375
GTCATAG19400.024.629171
CTAGTAC700.001372043724.5253033
TGTGTCG22750.024.45712570
Produced by FastQC (version 0.11.5)