FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758151

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758151
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1173135
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTACGC306.0224684E-446.43959848
TTACGCA404.7097354E-543.56727649
ACGCGAA459.2266375E-538.87117454
GTATCAA26300.035.8895031
GGTATCA12400.033.493011
GTGGTAT6300.032.144151
CTTATAC2900.030.7727181
CGTATGC1700.030.56681341
CTCGTAT1600.030.26001239
TGGTATC6950.030.1255872
TCAACGC31800.029.7901334
CAACGCA32250.029.3757115
ATCAACG32400.029.2384643
TATCAAC32400.029.0265922
AACGCAG33100.028.5188646
TCGTATG1700.028.50817340
TACGGAA1156.1126385E-428.16443470
TATACAC3500.027.458733
ACGCAGA35800.026.2687577
AGAGTAC35250.026.19178611
Produced by FastQC (version 0.11.5)