FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758153

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758153
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences419981
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5320.12667239708463002No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22650.043.6825031
GGTATCA8100.043.3840331
TATCAAC25500.038.2651632
ATCAACG26350.037.0352133
TCAACGC26600.036.687144
CAACGCA27150.036.0739145
AACGCAG27500.035.4906966
GTGGTAT2600.035.4297561
TGGTATC2850.034.7160952
CGAATTA601.4492198E-534.10941715
CGCAGAG29350.033.2457128
ACGCAGA29700.032.9688037
CAGAGTA30650.031.94312710
AGAGTAC30400.031.86917311
AGACGCG553.4611768E-431.03442825
GCAGAGT31600.030.7705699
GAGTACG17350.028.89962212
AGTACGG17750.028.24836213
ACGCGTA500.00812359527.32006327
CATAACC500.00816146727.2940332
Produced by FastQC (version 0.11.5)