FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758154

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758154
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences419981
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6530.15548322424109662No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4210.10024263002373916No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGATA200.007498794751.4985336
CTTACGC200.00771456851.1303752
GGTATCA9650.039.9295231
GTGGTAT3250.039.8697241
GTATCAA22450.039.6430551
TGGTATC3500.036.034742
TATCAAC25900.034.0869182
TCAACGC27450.032.015094
ATCAACG27350.032.0114143
CAACGCA27600.031.8410955
AACGCAG28550.030.9008946
GTACGGT450.00491687330.27783614
ACGCAGA30700.028.7368247
CGCAGAG30650.028.672578
AGAGTAC31100.028.2576911
CAGAGTA31700.027.93775410
GCAGAGT32100.027.4835039
GAGTACG17550.025.81379712
AGTACGG17700.025.40259213
TACGTTA700.001182102125.15399752
Produced by FastQC (version 0.11.5)