FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758155

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758155
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1067037
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39870.3736515228619064No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27230.2551926503017234No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27000.2530371486649479No Hit
GATTTTACACTGAGGATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTATTGCATGCAAGCTCTACAAATT25750.24132246585638548No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG25200.23616800542061805No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25120.23541826572087005No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23790.22295384321255962No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23280.21817425262666618No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21870.2049600904186078No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20480.1919333631354864No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA19680.18443596613800647No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19600.18368622643825847No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19500.18274905181357348No Hit
GTCCCTGACAGGTTCAGCGGCAGTGGATCAGGCACAGATTTTACACTGAG19140.1793752231647075No Hit
CTACAAATTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACG18360.17206526109216455No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT17410.16316210215765714No Hit
GGCCAAAAGTGTACGGAATTTGTAGAGCTTGCATGCAATAATAAACCCCA16720.15669559724733068No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16540.1550086829228977No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16380.15350920352340172No Hit
GTGTCTGGTGGCTCCATCACCGGTGGAGATTCCTACTGGAGTTGGCTCCG16000.14994793994959874No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15730.14741756846294926No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15720.14732385100048076No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCGCTGAACCTGTCAGGGACCCCG15620.14638667637579578No Hit
AGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGGTTCTAATCGGGCC14860.13926414922818983No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC14770.13842069206597335No Hit
GAATTTGTAGAGCTTGCATGCAATAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATCCTCAGTGTAAAAT14560.13645262535413485No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACCCAAATAGATCAGGAGTTGTGGAGACTGCCCTGGCTTCTGCAGGTA13610.12754946641962744No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13550.12698716164481644No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13510.12661229179494246No Hit
CACCTGCACTGTGTCTGGTGGCTCCATCACCGGTGGAGATTCCTACTGGA13330.12492537747050947No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCGGGAGAGCCGGCCTCCATCTCCT13170.12342589807101347No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13140.12314474568360798No Hit
GCAGTGGATCAGGCACAGATTTTACACTGAGGATCAGCAGAGTGGAGGCT13040.12220757105892298No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12980.12164526628411199No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12880.120708091659427No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12790.11986463449721049No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT12770.1196771995722735No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG12520.11733426301056102No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGCGATATTGTGATGACTCAGTCTCCACTCTCC12190.11424158674910054No Hit
GAGGATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTATTGCATGC12070.11311697719947857No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT12040.11283582481207306No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12020.11264838988713606No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGCGATATTGTGATGACTCAGTCTCCACT12010.11255467242466757No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11880.11133634541257706No Hit
GTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCTGA11830.11086775810023458No Hit
CTCCACAACTCCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCT11790.11049288825036058No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11640.10908712631333309No Hit
GTATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGGTTCTAAT11610.10880597392592758No Hit
GAGTGGAGGCTGAGGATGTTGGGGTTTATTATTGCATGCAAGCTCTACAAATTCCGTACACTTTTGGCCAGGGGA11600.10871225646345908No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG11540.10814995168864809No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11500.1077750818387741No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11480.10758764691383711No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC10990.10299549125288064No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10840.10158972931585315No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGCGATATTGTGATGA10710.10037140230376267No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2600.047.5500451
GGTATCA9800.042.051061
TGGTATC3150.040.339762
GTATCAA20600.036.008771
TCGTATG1102.7284841E-1134.5799140
CGTATGC1350.033.3274841
TATCAAC23200.032.1228372
TGTTAGA8700.031.85144270
ATCAACG24100.030.7821663
ACTTGCG450.00461499530.676937
TCAACGC24200.030.5144774
AACGCAG24750.029.8377766
CAACGCA24750.029.8363785
TATACAC2100.029.4385013
AGCCTAT1700.028.36380832
TTCGGGG7600.027.78020570
GAGCCTA1800.026.77923231
CGCAGAG27800.026.686518
AGAGTAC27600.026.6272611
CTTATAC2100.026.1651021
Produced by FastQC (version 0.11.5)