FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758157

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758157
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences903056
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27710.3068469729451994No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCA20520.22722843323116174No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18790.20807126025407063No Hit
CTCCTGCAGGGCCAGTCAGAGTGTCAGCACCAACTTAGCCTGGTATCAGCAGAAACCTGGCCAGGCTCCCAGGCT18540.20530288265622507No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16610.18393100760085754No Hit
TTGTTATACAGTTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC16560.18337733208128842No Hit
GTATAACAATTGGCCTCCCAGGTTCACTTTTGGCCGGGGGACCAGGCTGG16290.18038748427561524No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16050.17772984178168352No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15730.17418631845644122No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15450.17108573554685422No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15040.16654559628638757No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCGCCCTCTCCTGCAGGGCCAGTCAGAGTGTCAGCAC14720.16300207296114527No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCCTGGTCCCCCGGCCAAAAGTG14380.15923707942807536No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13950.154475469959781No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12440.13775446926879398No Hit
GTCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT12090.1338787406318102No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12010.13299285980049963No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCGCCCTCTCCTGCAGGGCCAGTCAGA11540.12778830991655002No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAACTGTATAACAATTGG11340.1255736078382736No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC11210.12413405148739391No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11080.12269449513651423No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10620.11760068035647844No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10330.11438936234297763No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCCTGGTCCCCCGGCCAAAAGTGAACCTGGGAGG10040.1111780443294768No Hit
TCTATGATGCATCCACCAGGGCCACTGGTGTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAATTCACT9740.10785599121206216No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGACACCAGTGGCCCTGGT9540.10564128913378573No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9510.10530908382204425No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT9300.10298364663985402No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9190.10176556049680197No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT9050.10021526904200846No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGC306.3336134E-445.966235
GTATCAA20400.041.1993371
GTGGTAT3350.040.96091
TATCAAC22800.036.862562
ATCAACG23650.035.392643
TCAACGC24000.034.8764954
CAACGCA24350.034.3770945
CCGTTAA400.002670026634.30285316
ACCGATC2450.033.6419127
AACGCAG25150.033.147186
CTTATAC2600.032.9853361
ACGAGAC2650.032.3683522
AGTATCG1101.0768417E-931.31445334
AGAGTAC26850.030.66176611
TATACAC2800.030.6292423
ACTAGAT450.00475228530.493113
ACGCAGA27650.030.1468097
CGCAGAG27550.030.0072158
CGATCAG2750.029.99017529
TAGGCAA2251.7771526E-929.87279570
Produced by FastQC (version 0.11.5)