FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758158

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758158
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences903056
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28640.3171453376091848No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22160.24538899027302846No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCA19980.22124873761981537No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17010.1883604117574104No Hit
TTGTTATACAGTTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC16800.18603497457522014No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16520.18293439166563313No Hit
CTCCTGCAGGGCCAGTCAGAGTGTCAGCACCAACTTAGCCTGGTATCAGCAGAAACCTGGCCAGGCTCCCAGGCT16270.18016601406778762No Hit
GTATAACAATTGGCCTCCCAGGTTCACTTTTGGCCGGGGGACCAGGCTGG15950.17662249074254532No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15250.16887103346857782No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14760.16344501337680056No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14750.1633342782728867No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCCTGGTCCCCCGGCCAAAAGTG14440.15990149005155826No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13720.15192856256976311No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCGCCCTCTCCTGCAGGGCCAGTCAGAGTGTCAGCAC12720.14085505217838096No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12680.1404121117627257No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAACTGTATAACAATTGG11970.13254991938484437No Hit
GTCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT11530.1276775748126362No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11470.1270131641891533No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11190.12391258127956628No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11100.12291596534434188No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCGCCCTCTCCTGCAGGGCCAGTCAGA10880.12047979305823782No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC10160.11250686557644266No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10130.1121746602647012No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10040.1111780443294768No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9930.10995995818642477No Hit
TCTATGATGCATCCACCAGGGCCACTGGTGTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAATTCACT9870.10929554756294182No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT9780.10829893162771745No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCCTGGTCCCCCGGCCAAAAGTGAACCTGGGAGG9720.10763452100423451No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT9690.10730231569249304No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9540.10564128913378573No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGACACCAGTGGCCCTGGT9440.1045339380946475No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTAT400.007235341751.9671170
GCGTAAG400.007235341751.9671170
GTATCAA20350.041.145911
TATCAAC23700.034.72382
ATCAACG23900.034.565213
CAACGCA24050.034.3496255
TCAACGC24150.034.2073944
GTAGTTT2054.7293724E-1033.79974770
AACGCAG24900.033.3147136
GTGGTAT3200.031.0992031
TGTTGCG450.004766330630.47471818
ATAAACG450.00477020530.4696583
GATGCTA1154.1199496E-430.12585870
CGCAGAG27850.030.0320328
GCAGAGT28150.029.5902049
AGAGTAC28200.029.53773911
ACGCAGA28250.029.3641177
CAGAGTA29400.028.33211710
ACGGGAC2250.025.9006416
AGTACGG23750.025.4020713
Produced by FastQC (version 0.11.5)