FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758159

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758159
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences635256
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT7120.1120807989220094No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC6460.10169128666238493No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGTT503.5768066E-451.60348570
TCGCTAT350.001368256439.3053413
GTATCAA11150.034.5491141
TCTATAC553.312647E-431.2705333
TCAACGC12750.029.946134
TATCAAC12950.029.746922
ATCAACG12800.029.5604213
TCGTATG605.371355E-428.79385640
CAACGCA13150.028.7736435
GTCTAGC605.517147E-428.6623951
AACGCAG13600.028.5803456
GTGGTAT3050.028.1925221
TAACACA809.7503E-525.7981854
AGAGTAC15500.025.29488611
CAGAGTA15550.025.21355210
CGCAGAG15650.024.6148628
CTATACT1001.5986112E-524.0783084
ACGCAGA16650.023.343077
GGTATCA8550.023.3321951
GCAGAGT17450.022.27299
Produced by FastQC (version 0.11.5)