FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758160

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758160
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences635256
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT7430.11696072134698451No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7180.11302530003652071No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12750.037.4570581
GTGGTAT2950.034.9404341
TATCAAC13650.034.7137682
CAACGCA14550.032.3101775
TTCGGGG2750.031.95512470
AACGCAG15150.031.2570656
TCAACGC15050.031.2367484
ATCAACG15000.031.1121063
AGAGTAC16600.028.94021211
CGCAGAG16550.028.6129658
CAGAGTA17000.028.05741310
ACGCAGA17100.027.8933357
GGTATCA7700.027.218751
GCAGAGT17600.026.9059419
TACGGGG7400.026.4337715
GTATACA951.083901E-525.316491
AGTACGG14900.024.87053113
GAGTACG15650.023.897912
GCCGTCG6250.021.6311670
GTACGGG17350.021.1607914
Produced by FastQC (version 0.11.5)