FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758161

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758161
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1334728
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT78800.5903824599468955No Hit
CTATTAAGCTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTCTC62010.46458903986430194No Hit
GATCTATGCTGCGTCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGAATCTGGGAGAGAATTCA57460.4304996973166068No Hit
GCTTAATAGTTACCCTATCACCTTCGGCCAAGGGACACGACTGGAGATTAAGCGAACTGTGGCTGCACCAT54320.4069743048770985No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG52690.3947620788655066No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC51030.38232508795799597No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC45760.342841387908248No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG44200.33115361332046683No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTAAGAGCATTAGCAGTTATTTAGCCTGGTATCAGCAAAGA44120.33055424026468316No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT43610.3267332370340624No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG42800.3206645848442529No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC42250.3165438950857403No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG40840.3059799449775535No Hit
GCATAGATCAGGAGCTGAGGGGCTTTCCCTGGTCTTTGCTGATACCAGGC40000.29968652789182515No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC39990.29961160625985217No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT39470.29571568139725846No Hit
GTCTCCATCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT39290.2943670920217452No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC38950.29181975653466474No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT37160.27840878441150557No Hit
GTGCAGCCACAGTTCGCTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG36530.2736887215972093No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC35040.26252539843323885No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT33520.25113731037334946No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG33350.2498636426298092No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT33000.24724138551075575No Hit
GACCAGGGAAAGCCCCTCAGCTCCTGATCTATGCTGCGTCCACTTTGCAA30340.2273122314059494No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG29730.22274201185559905No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG27640.20708339077325116No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC27160.20348715243854926No Hit
CCCTATCACCTTCGGCCAAGGGACACGACTGGAGATTAAGCGAACTGTGG26580.1991416977841178No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25530.1912749264269574No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTTC25460.1907504750031467No Hit
GTGATAGGGTAACTATTAAGCTGTTGACAGTAATAAGTTGCAAAATCTTC25410.19037586684328192No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25140.1883529827800121No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24700.185056430973202No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA24250.181684957534419No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG24090.1804862114228517No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA23460.17576614860855547No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23120.17321881312147494No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG22530.1687984368350705No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA22270.16685047440377365No Hit
GATCAGGAGCTGAGGGGCTTTCCCTGGTCTTTGCTGATACCAGGCTAAAT22180.16617617971601703No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT21910.16415329565274722No Hit
GTCGTGTCCCTTGGCCGAAGGTGATAGGGTAACTATTAAGCTGTTGACAGTAATAAGTTGCAAAATCTTCAGG21650.16220533322145037No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21390.1602573707901535No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT21200.1588338597826673No Hit
TCTATGCTGCGTCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGAATCTGGGAGAGAATTCACT20810.15591191613572203No Hit
GCTCCTGATCTATGCTGCGTCCACTTTGCAAAGTGGGGTCCCATCAAGGT20710.15516269981599246No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTGAATCTGGGAGAGAATTCACT20580.15418871860034403No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA20530.15381411044047927No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTCTCCCAGATTCACTGCCG20470.1533645806486415No Hit
ACTTATTACTGTCAACAGCTTAATAGTTACCCTATCACCTTCGGCCAAGGGACACGACTGGAGATTAAGCGAA20080.15044263700169622No Hit
GGACACGACTGGAGATTAAGCGAACTGTGGCTGCACCATCTGTCTTCATC20010.14991818557788553No Hit
CATCAAGGTTCAGCGGCAGTGAATCTGGGAGAGAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTTTG19520.14624702561121067No Hit
GTAAGAGCATTAGCAGTTATTTAGCCTGGTATCAGCAAAGACCAGGGAAAGCCCCTCAGCTCCTGATCTATGCTG19510.14617210397923772No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC18960.14205141422072512No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG18870.14137711953296853No Hit
GGGTAACTATTAAGCTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTG18860.14130219790099555No Hit
ATGCTGCGTCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGAATCTGGGAGAGAATTCACTCTCA18640.13965392199759052No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC17810.13343542654383514No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC17790.13328558327988924No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC17710.1326862102241056No Hit
ACCTTGATGGGACCCCACTTTGCAAAGTGGACGCAGCATAGATCAGGAGCTGAGGGGCTTTCCCTGGTCTTTG17690.1325363669601597No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC17500.1311128559526735No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA17310.12968934494518733No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC17020.12751661761797162No Hit
CCATCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG16880.1264677147703502No Hit
GGTATCAGCAAAGACCAGGGAAAGCCCCTCAGCTCCTGATCTATGCTGCGTCCACTTTGCAAAGTGGGGTCCCAT16820.12601818497851247No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT16550.12399530091524266No Hit
GCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTTCCTGTCTGCATCTGTAGGAGA16480.12347084949143196No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT16350.12249686827578354No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16130.1208485923723785No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA16040.1201742976846219No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT15920.11927523810094641No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC15790.11830125688529797No Hit
CTCACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACAGCTTAATAGTTACCCTATCAC15630.11710251077373067No Hit
GTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCA15420.1155291565022986No Hit
CGACTGGAGATTAAGCGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT15400.11537931323835268No Hit
GGGAAAGCCCCTCAGCTCCTGATCTATGCTGCGTCCACTTTGCAAAGTGG15370.11515454834243383No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTAAGA15320.11477994018256903No Hit
TAGTTACCCTATCACCTTCGGCCAAGGGACACGACTGGAGATTAAGCGAACTGTGGCTGCACCATCTGTCTTCAT15250.11425548875875834No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT15170.1136561157029747No Hit
GGTGATAGGGTAACTATTAAGCTGTTGACAGTAATAAGTTGCAAAATCTT15160.11358119407100173No Hit
GAGTCACCATCACTTGCCGGGCCAGTAAGAGCATTAGCAGTTATTTAGCCTGGTATCAGCAAAGACCAGGGAAAG15080.11298182101521809No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT14910.11170815327167782No Hit
CTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTC14880.11148338837575897No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA14800.1108840153199753No Hit
TCCCTGGTCTTTGCTGATACCAGGCTAAATAACTGCTAATGCTCTTACTGGCCCGGCAAGTGATGGTGACT14630.10961034757643504No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC14580.10923573941657026No Hit
CACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACAGCTTAATAGTTACCCTATCACCTT14360.10758746351316523No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC14310.10721285535330044No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14220.10653856066554385No Hit
CCTTCGGCCAAGGGACACGACTGGAGATTAAGCGAACTGTGGCTGCACCA14210.1064636390335709No Hit
CTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTAAGAGCATTAGC14180.10623887413765201No Hit
GAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACAGCTTAATAGTTAC14020.10504012802608471No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13960.10459059823424698No Hit
ACTGGAGATTAAGCGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGC13830.10361661701859853No Hit
CACCATCACTTGCCGGGCCAGTAAGAGCATTAGCAGTTATTTAGCCTGGTATCAGCAAAGACCAGGGAAAGCCC13800.10339185212267968No Hit
CAGCATAGATCAGGAGCTGAGGGGCTTTCCCTGGTCTTTGCTGATACCAGGCTAAATAACTGCTAATGCTCTTA13780.10324200885873376No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13770.10316708722676081No Hit
CAGCAAAGACCAGGGAAAGCCCCTCAGCTCCTGATCTATGCTGCGTCCAC13700.10264263580295012No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA13550.10151881132335577No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC13450.1007695950036262No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG13440.10069467337165325No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTACGC200.00731063251.8368840
GTATCAA25050.040.1105651
CTTATAC2000.039.4363561
TCGACGT1850.038.98795329
CTGATAG400.001560562638.27020668
CGACGTC1900.037.97905730
TCGTATG1100.037.6995540
ACTCGAC1950.036.9719227
TATCAAC27300.036.427912
AGACTCG2100.035.9510525
CTCGACG2150.035.13869528
ATCAACG28450.034.9554253
TCAACGC28850.034.7098054
CAACGCA29300.034.2963335
AACGCAG29850.033.664416
GTGGTAT3300.033.2533151
CGTATGC1253.6379788E-1233.1994241
ACGAGAC2300.032.80764822
GACTCGA2300.031.34223226
CGCAGAG32200.031.2016898
Produced by FastQC (version 0.11.5)