FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758162

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758162
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1334728
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT78240.58618684855641No Hit
CTATTAAGCTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTCTC63940.4790489148350825No Hit
GATCTATGCTGCGTCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGAATCTGGGAGAGAATTCA56490.4232322990152301No Hit
GCTTAATAGTTACCCTATCACCTTCGGCCAAGGGACACGACTGGAGATTAAGCGAACTGTGGCTGCACCAT50290.37678088719199715No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT48480.36322007180489213No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC48450.3629953069089732No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG46460.34808590214635493No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC46020.34478935033954483No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41670.31219844043130884No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG40470.3032078445945541No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTAAGAGCATTAGCAGTTATTTAGCCTGGTATCAGCAAAGA38380.28754922351220624No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT38270.28672508556050375No Hit
GTGCAGCCACAGTTCGCTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG36680.27481254607680367No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC36470.2732391918053716No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC36060.2701674048944804No Hit
GTCTCCATCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT35950.2693432669427778No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC35410.2652974988162382No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG35160.26342445801691433No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT34940.2617761821135093No Hit
GCATAGATCAGGAGCTGAGGGGCTTTCCCTGGTCTTTGCTGATACCAGGC34600.25922884662642875No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT32090.24042351700121672No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT31010.23233198074813746No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT30680.2298595668930299No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG30120.22566395550254437No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29950.22439028775900408No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG27360.2049855850780084No Hit
GACCAGGGAAAGCCCCTCAGCTCCTGATCTATGCTGCGTCCACTTTGCAA27280.20438621202222476No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA26540.19884201125622597No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA26450.19816771656846938No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26380.1976432651446587No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26260.1967442055609832No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC26090.19547053781744295No Hit
GTGATAGGGTAACTATTAAGCTGTTGACAGTAATAAGTTGCAAAATCTTC26060.1952457729215241No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTGAATCTGGGAGAGAATTCACT25240.18910219909974166No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTTC25010.18737900156436368No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG24920.18670470687660706No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24800.1858056472929316No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG23290.1744924808650152No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23150.17344357801739382No Hit
CCCTATCACCTTCGGCCAAGGGACACGACTGGAGATTAAGCGAACTGTGG22950.17194514537793468No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT22600.1693228882588812No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA22340.16737492582758434No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTCTCCCAGATTCACTGCCG21820.16347900096499063No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT21800.1633291577010447No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG21760.16302947117315286No Hit
GTCGTGTCCCTTGGCCGAAGGTGATAGGGTAACTATTAAGCTGTTGACAGTAATAAGTTGCAAAATCTTCAGG21670.16235517648539627No Hit
GGACACGACTGGAGATTAAGCGAACTGTGGCTGCACCATCTGTCTTCATC21050.15771003530307298No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA20850.15621160266361386No Hit
GGGTAACTATTAAGCTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTG20190.15126677495339874No Hit
GATCAGGAGCTGAGGGGCTTTCCCTGGTCTTTGCTGATACCAGGCTAAAT19550.14647179050712955No Hit
ACTTATTACTGTCAACAGCTTAATAGTTACCCTATCACCTTCGGCCAAGGGACACGACTGGAGATTAAGCGAA19540.1463968688751566No Hit
GCTCCTGATCTATGCTGCGTCCACTTTGCAAAGTGGGGTCCCATCAAGGT19390.14527304439556224No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19340.14489843623569748No Hit
TCTATGCTGCGTCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGAATCTGGGAGAGAATTCACT18720.14025329505337417No Hit
CATCAAGGTTCAGCGGCAGTGAATCTGGGAGAGAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTTTG18490.1385300975179962No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18370.1376310379343207No Hit
ATGCTGCGTCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGAATCTGGGAGAGAATTCACTCTCA18230.1365821350866993No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC17930.13433448612751062No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC17780.13321066164791628No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC17590.13178715064043012No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC17530.13133762084859235No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC17460.13081316942478166No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA17270.12938965841729552No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA17250.1292398151533496No Hit
ACCTTGATGGGACCCCACTTTGCAAAGTGGACGCAGCATAGATCAGGAGCTGAGGGGCTTTCCCTGGTCTTTG16340.12242194664381058No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC15910.11920031646897346No Hit
GGTGATAGGGTAACTATTAAGCTGTTGACAGTAATAAGTTGCAAAATCTT15830.1186009434131898No Hit
CCATCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG15770.11815141362135208No Hit
GTAAGAGCATTAGCAGTTATTTAGCCTGGTATCAGCAAAGACCAGGGAAAGCCCCTCAGCTCCTGATCTATGCTG15720.11777680546148728No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA15470.11590376466216337No Hit
CTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTC15340.11492978344651494No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15330.11485486181454199No Hit
GGTATCAGCAAAGACCAGGGAAAGCCCCTCAGCTCCTGATCTATGCTGCGTCCACTTTGCAAAGTGGGGTCCCAT15140.11343135080705581No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT15140.11343135080705581No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15100.11313166427916399No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT14940.11193291816759669No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT14820.11103385858392122No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14740.11043448552813756No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14740.11043448552813756No Hit
CGACTGGAGATTAAGCGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT14720.11028464226419164No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC14680.10998495573629984No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG14530.10886113125670549No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG14440.10818683656894888No Hit
CTCACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACAGCTTAATAGTTACCCTATCAC14310.10721285535330044No Hit
GTATCAACGCAGAGTACGGGAGTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT14210.1064636390335709No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14060.10533981455397655No Hit
GAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACAGCTTAATAGTTAC14000.1048902847621388No Hit
TCCCTGGTCTTTGCTGATACCAGGCTAAATAACTGCTAATGCTCTTACTGGCCCGGCAAGTGATGGTGACT13970.10466551986621994No Hit
ACTGGAGATTAAGCGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGC13940.10444075497030106No Hit
GTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCA13910.10421599007438219No Hit
GCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTTCCTGTCTGCATCTGTAGGAGA13870.10391630354649037No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA13820.10354169538662558No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA13720.10279247906689604No Hit
GGCCGAAGGTGATAGGGTAACTATTAAGCTGTTGACAGTAATAAGTTGCA13680.1024927925390042No Hit
CTCCTGATCTATGCTGCGTCCACTTTGCAAAGTGGGGTCCCATCAAGGTT13650.10226802764308532No Hit
GGGAAAGCCCCTCAGCTCCTGATCTATGCTGCGTCCACTTTGCAAAGTGG13640.10219310601111238No Hit
CACCATCACTTGCCGGGCCAGTAAGAGCATTAGCAGTTATTTAGCCTGGTATCAGCAAAGACCAGGGAAAGCCC13420.10054483010770733No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC13410.10046990847573439No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3250.042.2246131
GTATCAA26850.040.1212461
TATCAAC29700.036.135382
ATCAACG30400.034.8273473
TCAACGC30450.034.766254
CAACGCA30650.034.317125
AACGCAG31100.034.0408976
CGCAGAG33550.031.4529328
AGAGTAC34350.031.31885511
ACGCAGA34600.030.5974567
CAGAGTA35200.030.46524210
GCAGAGT35250.029.936059
TGTTAGA13450.029.85630670
TACGGGT2000.029.127515
GAGTACG27250.027.91085412
AGTACGG27350.027.80880413
ACGGTCT500.00746812327.7995347
GATCGGT500.00800196927.40898511
ATGTGCG2150.025.73583641
CGAACTA1359.904397E-925.43777329
Produced by FastQC (version 0.11.5)