FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758166

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758166
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences729362
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11520.1579462598819242No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18600.039.7138371
TATCAAC21700.033.5242
TCAACGC21850.032.9548954
CAACGCA22250.032.8247685
ATCAACG22000.032.734693
GGTATCA7600.032.548821
AACGCAG22550.032.3880736
CGCAGAG24800.029.7261648
ACGCAGA24700.029.5688727
AGAGTAC24800.028.75833511
CAGAGTA24950.028.5854410
GCAGAGT26650.027.5339589
GTGGTAT3900.026.428531
TGGTATC4200.024.5104712
AGTACGG21600.024.44663413
GAGTACG22050.023.79221712
GTGCTAA1507.6224933E-720.6142541
GTATAGA1004.45576E-420.6142541
TAATACG850.004267824320.1698744
GTGCTAT1206.590044E-520.0416361
Produced by FastQC (version 0.11.5)