FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758168

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758168
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences942153
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14480.15369053646276135No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29400.038.7520031
TATCAAC33400.033.457232
GGTATCA13150.033.4031641
ATCAACG34100.032.955723
TCAACGC34450.032.4168324
AACGCAG35100.032.013416
CAACGCA35150.031.967875
TAACGAA450.00436353431.02982350
ACGCAGA37300.030.1252177
CGATTTG605.581098E-428.60775628
CGCAGAG39350.028.4687398
AGAGTAC38750.028.37909511
CAGAGTA39350.027.94637710
GCTTAGG1254.3928594E-927.4532261
GTGGTAT5750.026.8564171
GCAGAGT42100.026.8532649
ATTCGTC658.1601634E-426.80778947
CCGATAA658.1936765E-426.78898646
AACGAAC2200.025.41415251
AGTCGAC700.001150206925.27641963
Produced by FastQC (version 0.11.5)