FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758170

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758170
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences562693
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8680.1542581834144018No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24200.043.454711
GTGGTAT3200.041.7473071
TATCAAC26550.039.465382
CAACGCA28650.036.4466325
ATCAACG28700.036.386373
AACGCAG28800.036.3756836
TCAACGC28700.036.144564
ACGCAGA30900.033.903557
CGCAGAG30750.033.623598
GGTATCA9350.033.338311
AGAGTAC30400.032.88451811
CAGAGTA31550.032.01141410
GCAGAGT32600.031.4004469
ACGGGTA1052.5025656E-829.35030416
GAGTACT12700.028.84445212
AGTACTT13100.027.96370313
TGGTATC5000.027.3936182
GAGTACG23400.027.06687412
AGTACGG23400.026.77425813
GTAGTTT2206.304799E-826.67656170
Produced by FastQC (version 0.11.5)