FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758171

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758171
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97261
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC252.5111868E-455.39499741
ATGCCGT306.079615E-446.30721344
TATGCCG306.11102E-446.25887343
TCGTATG306.190096E-446.13847440
CACGAGA306.564381E-445.59259421
CCCACGA306.564381E-445.59259419
TATACAC405.251854E-542.721083
GTATCAA2600.042.063831
TACACAT504.060881E-641.0122385
GCCGTCT350.001278538839.8167146
TGCCGTC350.001281840139.79585645
GACCGAG350.001401719939.07936526
AGACCGA350.001401719939.07936525
CGAGCCC350.00140527139.05927315
TATCAAC3000.036.4553222
GTAACAA400.002190791535.6811662
ACGAGAC400.002699952634.19444722
TCCGAGC400.00270677634.17686513
CGGGGAG400.00270677634.17686517
TCAACGC3400.033.171664
Produced by FastQC (version 0.11.5)