FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758175

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758175
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2469154
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36260.14685191770136655No Hit
CTATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCGGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC30280.12263309619408105No Hit
GTATAATAGTTACACTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCAT28420.1151001517118819No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24920.10092525618086196No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA47250.035.7826731
GTGGTAT10550.032.3128931
TATCAAC54300.031.00942
ACCGTAC1800.030.62762827
ATCAACG56550.029.9582773
TCAACGC56700.029.7593694
CAACGCA57700.029.24425
AACGCAG58850.028.6147986
TCGTATG2350.026.52035140
ACGCAGA63900.026.3485747
AGAGTAC66850.025.13281611
CGCAGAG69000.024.5008728
CAGAGTA69350.024.12897910
GCAGAGT72850.023.158789
CTCGTAT2450.022.5971939
CGTACTA2500.022.06262429
GTACTAG2700.021.7112930
CGTATGC2700.020.5299341
AGTACGG55750.020.31684113
GAGTACG56650.019.93369912
Produced by FastQC (version 0.11.5)