FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758176

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758176
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2469154
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36980.14976789621060493No Hit
CTATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCGGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC30150.12210660007435745No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28640.11599114514526028No Hit
GTATAATAGTTACACTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCAT25940.10505622573561632No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA52000.036.0786481
GTGGTAT11050.035.202481
TATCAAC58200.031.5628412
TCAACGC60300.030.3905814
ATCAACG60600.030.301163
CAACGCA60950.030.066485
AACGCAG63300.029.0045836
ACGCAGA68300.026.9819417
AGAGTAC70650.026.22991611
CGCAGAG72250.025.5068058
CAGAGTA73300.025.23472810
GCAGAGT76650.024.1771859
AGTACGG60000.022.63425313
GAGTACG62150.022.07252912
GGTATCA45350.020.6466081
CTATACG1409.631678E-619.6467654
TGTTAGA9400.019.45605970
TTAACGG3001.8189894E-1218.4126735
GTACGGG73100.018.34288214
GAGCAAA26600.018.1708370
Produced by FastQC (version 0.11.5)