FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157907
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG408.2012775E-751.76633540
AACGTAA200.00749735551.48598535
AATGCGA200.007678457551.1762218
CTCGTAT352.2681425E-549.26970739
GTGGTAT1150.047.4828341
GTATCAA8950.043.470671
ACGAGAC651.2409146E-842.00404422
TATCTCG503.940995E-641.21504636
TATGCCG602.7055285E-740.5877243
GTATGCC602.774923E-740.4570942
CGTATGC602.831548E-740.35318441
ACGGACT702.359593E-839.05323827
GCCGTCT654.9158916E-737.6113246
TATCAAC10350.037.2608342
TCTCCGA655.890797E-736.7535411
GGTATCA3550.036.531691
CGGACTC754.3331966E-836.44968828
ATCTCCG754.3817636E-836.40350310
ATCAACG10600.036.3820423
TCAACGC10800.035.70834
Produced by FastQC (version 0.11.5)