FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758179

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758179
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1200665
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAT602.8852628E-740.29539
TATCGTA602.924753E-740.22524638
GTATCAA47050.034.4790841
GGTATCA23150.033.348161
TCGTATG1500.032.27832440
TATCAAC56000.029.2734622
ATCAACG57350.028.7029823
AACGCAG57000.028.7026166
CTTATAC3350.028.4256381
TCAACGC57850.028.2797074
CAACGCA58400.028.1310255
GCGAATA907.2977873E-626.64359533
ACGCAGA61650.026.5917637
AGAGTAC62100.025.95874811
CAGAGTA62850.025.65004510
CGCAGAG64300.025.4958368
GACTAAG3400.025.03390526
GCAGAGT66750.024.5590119
TAAGGCG3200.024.51868429
CGTATGC1851.8189894E-1124.34758241
Produced by FastQC (version 0.11.5)