FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758181

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758181
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences850548
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATACAGCGCCAACAATGAGAACTACTTAGCTTGGTACCAGCAGAA42450.49908999844805935No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT29430.34601221800533305No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19730.23196809586290248No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18930.2225623950676505No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17270.20304556591750259No Hit
CTCCAAAACTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT16710.1964615753608262No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16680.19610886158100424No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16070.1889370147246246No Hit
ATATTATTCTACTCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT15660.18411659306705797No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15410.1811773115685417No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC15220.17894345762966934No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC14940.17565146235133114No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14610.17177161077328967No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA13910.1635416225774442No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13560.15942662847952144No Hit
GTAATATACAGCCGTGTCTGCGGCGGTAACAGAGTTCAGCTGCAGGGAGA13370.1571927745406491No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12930.1520196391032605No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATA12850.15107906902373527No Hit
GTTTTGGAGGCTGTCCTGCTTTCTGCTGGTACCAAGCTAAGTAGTTCTCATTGTTGGCGCTGTATAAAACACT12790.15037364146409138No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12380.14555321980652475No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11570.1360299477513321No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11280.13262038121305322No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC11210.1317973823934687No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10780.12674181821602074No Hit
GAATAATATTGTTGACAGAAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC10670.12544853435667358No Hit
AAGCAGGACAGCCTCCAAAACTGCTCATTTACTGGGCATCTACCCGGGAA10360.12180382529851343No Hit
GTATATTACTGTGCGAGAACAACGACAACATATTATTATGCTTCGGGCAG10130.11909968631987847No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC10060.11827668750029392No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9930.11674826112106548No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9730.11439683592225247No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC9560.11239812450326142No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGCGCCAA9220.10840070166527932No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9220.10840070166527932No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9080.10675470402611023No Hit
CTACTTAGCTTGGTACCAGCAGAAAGCAGGACAGCCTCCAAAACTGCTCATTTACTGGGCATCTACCCGGGAATC9010.10593170520652567No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTTCTGTCAACAATATTATTCTACT8810.10358028000771266No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8650.10169913984866227No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8580.10087614102907771No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTTCTGTCAACAATATTATTCTACTCCGACGTTCGG8550.10052342724925578No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22350.041.8757321
GGTATCA8400.041.629211
CACGGAT4500.041.41814470
ACTCGAC1450.040.2434127
TCGTATG1009.094947E-1238.03541640
TATCAAC25200.037.4118732
TCGACGT1600.036.48347529
GACGTCA1750.035.34134731
ATCAACG26550.035.251323
TCAACGC26900.034.920114
CAACGCA27200.034.6615
CGTATGC1102.7284841E-1134.62076641
AACGCAG27650.034.0968976
CTTATAC2050.033.446711
CGACGTC1750.033.3602530
CTCGACG1750.033.35435528
CTCGTAT851.1934571E-732.5147539
ACGGGTT1002.8218687E-432.13476670
TATACAC2550.030.9218143
GGCGGTA3550.030.90832722
Produced by FastQC (version 0.11.5)