FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758182

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758182
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences850548
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATACAGCGCCAACAATGAGAACTACTTAGCTTGGTACCAGCAGAA35360.4157319751501385No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28570.33590108965043713No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21860.2570107742302609No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19890.2338492360219529No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18410.21644868955073668No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC17280.20316313717744322No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16170.1901127273240311No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15630.183763879287236No Hit
CTCCAAAACTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT15400.18105974030860106No Hit
ATATTATTCTACTCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT14250.1675390454154263No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14180.16671604659584174No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14090.1656579052563759No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13740.16154291115845315No Hit
GAATAATATTGTTGACAGAAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC13500.15872120091987754No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC13390.1574279170605304No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13270.1560170619412426No Hit
GTAATATACAGCCGTGTCTGCGGCGGTAACAGAGTTCAGCTGCAGGGAGA12730.14966821390444748No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12710.14943307138456619No Hit
GTTTTGGAGGCTGTCCTGCTTTCTGCTGGTACCAAGCTAAGTAGTTCTCATTGTTGGCGCTGTATAAAACACT12300.14461264972699955No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11850.13932194302967027No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA11090.13038652727418087No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10970.12897567215489308No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10770.12662424695608007No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGGT10700.1258012481364955No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATA10480.12321468041780123No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC10300.12109839773886953No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9970.11721854616082808No Hit
GTATATTACTGTGCGAGAACAACGACAACATATTATTATGCTTCGGGCAG9890.11627797608130287No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9780.11498469222195572No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC9630.11322112332284597No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9610.11298598080296468No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9130.10734256032581346No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9100.10698984654599153No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9060.10651956150622892No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT8970.10546142016676306No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTTCTGTCAACAATATTATTCTACT8780.10322756622789073No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8750.10287485244806878No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT8590.10099371228901838No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA8550.10052342724925578No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGAT3800.055.1409370
CGAATCG200.00760432651.3214314
GTATCAA24400.041.7122881
TATCAAC26700.037.5835762
TCAACGC27100.036.7393264
ATCAACG27450.036.5309073
CAACGCA27650.036.0085265
AACGCAG28450.034.8757176
CCACGGA4150.034.7552369
GGTATCA9650.034.091121
CGGTACC958.038114E-932.9855945
AGAGTAC30600.032.87254711
CGCAGAG30750.032.4896628
GTGGTAT3950.032.0997771
CAGAGTA31600.031.83227710
GCAGAGT32200.031.3453859
TTGGACG4050.031.3204659
GGCGGTA3850.031.12585422
ACGCAGA31900.030.9966437
CGGTAAG450.004806985630.42163815
Produced by FastQC (version 0.11.5)