FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758184

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758184
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3745726
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT51490.13746333821534196No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG48030.1282261436100772No Hit
GTATTACTGTCAGCAGTATGATAGGTCTCTCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGG40180.1072689246357048No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA83350.037.45411
GGTATCA37000.037.3957251
TATCAAC92900.033.274552
TCAACGC94950.032.502134
ATCAACG96000.032.078043
CAACGCA97700.031.6220345
AACGCAG100150.031.0200236
GTGGTAT17800.029.0047381
ACGCAGA109550.028.3897047
AGAGTAC109800.028.10597411
CGCAGAG113550.027.3896278
CAGAGTA116150.026.74692310
GCAGAGT117300.026.4554029
TGGTATC19400.025.3455962
AGTACGG83350.022.75895713
GAGTACG86000.022.217512
GAGTACT53600.022.05527712
AGTACTT55600.021.07649613
GTACGGG100350.018.90342714
CGTTCGG4550.018.88802719
Produced by FastQC (version 0.11.5)