FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758187

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758187
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1110816
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT1150.045.1092439
GTATCAA31150.042.110371
ACGCGTA1750.039.1858527
GGTATCA12700.036.9458051
TCGTATG1700.034.6199540
TATCAAC39150.033.505442
TCAACGC39700.032.782454
CAACGCA40300.032.1272965
ATCAACG40750.031.9377483
AACGCAG41800.030.9757966
GACGCGT2350.029.17306726
AGACGCG2350.029.16780925
ACGCAGA44800.028.898927
AGAGTAC45000.028.6943711
GTGGTAT4900.028.6573521
ACGAGAC2400.028.54600122
CGCAGAG45650.028.4358548
CAGAGTA46200.027.87492410
CGTATGC2000.027.7376141
TATGCCG2200.026.83971443
Produced by FastQC (version 0.11.5)