FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758188

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758188
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1110816
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19050.17149554921787227No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA32700.039.298241
GGTATCA14300.036.185161
TATCAAC39250.032.716592
TCAACGC39950.031.947444
AACGCAG40300.031.8412276
CAACGCA40350.031.7169655
ATCAACG40950.031.2578753
ACGCAGA42600.030.0417757
CGCAGAG44850.028.6872528
AGAGTAC43750.028.54817611
CAGAGTA44400.028.1302410
TTAGCGC500.00788866627.48880431
ATACCGC500.00805003327.3749666
GCAGAGT47250.027.012869
GTGGTAT6400.025.1656111
AGTACGG34400.023.17615913
AGTACTT19800.022.98425713
GAGTACG34700.022.9757912
TAGCGCC750.002033880722.91976732
GAGTACT19950.022.46841412
Produced by FastQC (version 0.11.5)