FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758190

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758190
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2233549
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50850.22766458224108807No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA77500.041.6115841
GGTATCA31900.037.6687771
TATCAAC88250.036.3631252
ATCAACG92000.035.0425223
TCAACGC92250.034.9791374
CAACGCA93900.034.3280835
AACGCAG97100.033.1967776
GTGGTAT11500.031.853051
CGCAGAG104450.030.8607658
ACGCAGA104750.030.8376487
AGAGTAC106550.030.06004111
CAGAGTA107900.029.74730510
TGGTATC12050.029.2430882
GCAGAGT110950.029.1144089
GAGTACG68650.026.3401712
AGTACGG68150.026.33279213
GAGTACT59200.023.21174412
AGTACTT59150.023.17357613
GTACGGG77650.022.5388614
TACGGGG31200.022.46114515
Produced by FastQC (version 0.11.5)