FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758192

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758192
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1025405
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32770.3195810435876556No Hit
GTATAATAACTGGCCTCCGTACATTTTTGGCCAGGGGACCAAGCTGGAGATCAACCGAACTGTGGCTGCACCAT29490.28759368249618444No Hit
GTAATACACAGCCGTGTCTGCGGCGGTCACAGATCTCAGCTCCAGGGAGA28300.2759885118562909No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT25970.25326578278826417No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT25090.24468380786128408No Hit
GTTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGCTGAGAGTGAACT24790.24175813459072268No Hit
GTCTCTGGTGCCTCCATCAGCAGCAAGACTTACTACTGGGCCTGGATCCG22900.22332639298618595No Hit
GTGTATTACTGTGCCAGGACTAATATGTGGAAGTTATTCATTACTTATTGGTTCGACCCCTGGGGCCAGGGAATC22620.22059576460032865No Hit
GTCAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA20250.19748294576289369No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19480.18997371770178612No Hit
GTCTGGGACAGAGTTCACTCTCAGCATCAGCAGCCTGCAGTCTGAAGATT19410.1892910606053218No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT18440.17983138369717333No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18330.1787586368313008No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18070.1762230533301476No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC17940.174955261579571No Hit
GTGCAGCCACAGTTCGGTTGATCTCCAGCTTGGTCCCCTGGCCAAAAATGTACGGAGGCCAGTTATTATACTG17840.17398003715605054No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC17760.17319985761723417No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17580.17144445365489733No Hit
CACCTGCACTGTCTCTGGTGCCTCCATCAGCAGCAAGACTTACTACTGGG17410.16978657213491255No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG17400.1696890496925605No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT17070.166470809094943No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16760.16344761338202954No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16740.16325256849732545No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG16630.16217982163145295No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT15480.1509647407609676No Hit
GAGTTCACTCTCAGCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATAATAACTGG15320.1494043816833349No Hit
GTCTATTATAGTGGGAGCACCTATTACAACCCGTCTCTCAAGAGTCGAGT15250.14872172458687055No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATCAGCAG15000.1462836635280694No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA14860.14491834933514075No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14690.14326046781515597No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA14390.14033479454459458No Hit
CTCCTGCAGGGCCAGTCAGAGTATCAGCAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT14260.13906700279401799No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14140.13789673348579343No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13770.13428840311876772No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT13650.13311813381054316No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC13230.1290221912317572No Hit
GAGTATCAGCAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATC13080.12755935459647652No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC12800.12482872621061923No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT12750.12434111399885897No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA12690.1237559793447467No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT12550.12239066515181807No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA12410.12102535095888942No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC12380.12073278363183328No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12330.12024517142007304No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT12270.11966003676596078No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12240.11936746943890462No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG12190.1188798572271444No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT12140.11839224501538417No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT12060.1176120654765678No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATCAGCAGCAACTTAGCCTGGTA11990.11692940838010346No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT11560.11273594335896547No Hit
CTATTATAGTGGGAGCACCTATTACAACCCGTCTCTCAAGAGTCGAGTCACAATTTCCGCAGACACGTCCAAGGA11390.11107806183898071No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA11320.11039540474251637No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC11300.11020035985781229No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA11130.1085424783378275No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10930.10659202949078657No Hit
GTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCA10830.1056168050672661No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA10720.10454405820139359No Hit
CAGTAATACACAGCCGTGTCTGCGGCGGTCACAGATCTCAGCTCCAGGGA10720.10454405820139359No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10650.10386140110492928No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT10460.10200847470024038No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10450.10191095225788833No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCA10430.10171590737318426No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC10370.10113077271907196No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACTA350.00429071259.30462370
GTATCAA22350.040.091091
GTTACGC400.001661922237.78222768
GGTATCA9200.037.316171
TATCAAC24950.036.1584432
TGCGGAA11450.033.83902770
ATCAACG26450.033.8369453
TCAACGC26600.033.5155874
CAACGCA26850.033.2035265
AATGGTA1052.437742E-432.9470170
AACGCAG27200.032.9023326
AGTTACG450.003678229232.13220267
AGAGTAC28850.030.66251811
CCGGAAT12000.030.55835370
TCTACGA450.00475417130.491032
CGCAGAG29450.030.2721428
TAGGCAA2352.077286E-929.4420170
GCAGAGT30300.029.309769
ACGCAGA32100.027.9866777
GTGGTAT5600.027.587311
Produced by FastQC (version 0.11.5)