FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758196

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758196
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85101
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1370.16098518231278128No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTTAG100.00895621133.9562770
TCACGGT200.00721460151.96785742
GTATAGA200.007560505551.356471
ATCAATC200.007578118751.3262827
TTGAAGG200.00759576251.296123
GGTATCA1300.050.039641
TGGTATC352.3673485E-548.8821682
CTGTGTC306.556942E-445.5965549
GTATCAA3650.043.1488151
TATCAAC4100.039.2249642
GTGGTAT451.03945255E-438.0418321
TCAACGC4300.037.378574
CAACGCA4400.036.5290575
ATCAACG4400.036.5290573
AACGCAG4550.035.3248026
CGCAGAG4850.033.1397638
ACGCAGA4950.032.4702727
AGAGTAC5200.029.59391611
CAGAGTA5250.029.31206910
GCAGAGT5500.029.2232469
Produced by FastQC (version 0.11.5)