FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758198

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758198
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127348
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3260.2559914564814524No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAGCGG200.00758435151.3303226
GTATACC200.00758435151.330321
TTCTGAC200.007619805651.26983616
GTATCAA6400.044.914031
TATCAAC7350.039.5277632
GTCTAGC350.001398163639.1088141
CTTTATG350.001406295739.06273315
GTGGTAT901.3096724E-1038.0224571
GGTATCA2250.036.501561
ATCAACG8000.036.316133
TGGTATC952.382876E-1035.978832
ATAGCGG400.002243006135.51918463
CGAACCT400.002362329735.14620253
TCAACGC8350.034.79394
ATCGTTG400.002520961734.68393740
AACGCAG8400.034.5867966
CAACGCA8450.034.3821375
TACTCCG601.4210978E-534.179895
CGCAGAG8750.033.203328
ACGCAGA8850.032.8281447
Produced by FastQC (version 0.11.5)