EGAF00005758199 FastQC Report

Basic Statistics

Measure	Value
Filename	EGAF00005758199
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	17652
Sequences flagged as poor quality	0
Sequence length	20-76
%GC	51

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT	227	1.2859732608203036	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	49	0.27758894176297305	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	44	0.24926353954226152	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35	0.19827781554498072	No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT	29	0.1642873328801269	Illumina PCR Primer Index 10 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	29	0.1642873328801269	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27	0.15295717199184228	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25	0.14162701110355766	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25	0.14162701110355766	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23	0.13029685021527304	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22	0.12463176977113076	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22	0.12463176977113076	No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATATCGTATGCCGT	21	0.11896668932698845	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20	0.11330160888284614	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20	0.11330160888284614	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20	0.11330160888284614	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19	0.10763652843870383	No Hit
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCT	19	0.10763652843870383	Illumina PCR Primer Index 10 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	19	0.10763652843870383	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19	0.10763652843870383	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19	0.10763652843870383	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18	0.10197144799456152	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18	0.10197144799456152	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGGGGG	50	6.1172614E-9	102.614174	70
GTGGTCG	15	0.0011991763	81.34832	59
GGTGGTC	20	3.6306497E-5	81.34831	58
AAGGGGG	35	2.0946973E-6	72.83702	69
CTTATAC	45	1.6370905E-11	61.529743	1
TTCTGCT	45	9.726136E-9	61.207726	52
GTCTTCT	45	1.0304575E-8	60.767384	49
CTTGAAA	55	5.475158E-10	58.941658	57
TATGCCG	45	1.7955244E-8	56.689037	43
TCGTATG	50	7.9853635E-10	56.59935	40
GTATGCC	45	1.9970685E-8	55.93819	42
CGTATGC	45	2.1611413E-8	55.387978	41
CTTCTGC	50	2.2346285E-8	55.086956	51
TGAAAAA	60	1.1459633E-9	54.232212	59
CCGTCTT	50	2.8076101E-8	53.53521	47
CGGCTTC	20	0.006712434	52.718445	39
GGGGGGG	65	3.0106254E-4	52.622658	70
AAAGGGG	35	3.4743693E-4	51.499702	68
CGCTAAT	45	4.04998E-8	51.191914	32
AATCTCG	40	8.884017E-7	50.74766	36

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content