FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758200

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758200
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17652
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6153.4840244731475187No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT520.29458418309539997No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT450.2549286199864038No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT440.24926353954226152No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT400.22660321776569228No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT340.19261273510083843No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT280.1586222524359846No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260.14729209154769998No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT250.14162701110355766No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230.13029685021527304No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT220.12463176977113076No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT220.12463176977113076No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT220.12463176977113076No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT190.10763652843870383No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT180.10197144799456152No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180.10197144799456152No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCTGG300.001404205577.7922670
CGCCTGC200.006688602352.76990541
AGAGTAC3300.028.44836411
CAGAGTA3350.028.02376410
GTATCAA3150.027.89521
AACGCAG3350.027.0896386
GCAGAGT3350.027.0896389
TATCAAC3500.026.8989452
ACGCAGA3400.026.691267
GAGTACT1555.456968E-1226.2459112
ATCAACG3600.026.077673
TCAACGC3650.025.720444
CAACGCA3650.025.720445
AGTACTT1607.2759576E-1225.42572613
GTACTTT1651.0913936E-1124.65524914
TACTTTT1651.0913936E-1124.65524915
CGCAGAG3700.024.5271038
ACTTTTT1701.8189894E-1123.93009416
CTTTTTT1752.5465852E-1123.2463817
TACGGGG952.07971E-523.0581515
Produced by FastQC (version 0.11.5)