FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005758201

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758201
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3137737
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGACCCACTACCTGCATGTGAGCAG220240.7019071388073634No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC198830.6336732492238834No Hit
CTCCTGGACAGTCGATCACCATTTCCTGCACTGGAACCAGCAATGATATT141670.4515037429841953No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT120920.3853732801697529No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATTTCCTG108970.3472885076091463No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG103650.33033361304660014No Hit
CACTAAGAGGCCCTCAGGGATTTCTAATCGGTTCTCTGGTTCCAAGTCTGGCAACACGGTCTCCCTGAAAATCT100650.32077258227824706No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTC95750.30515623202327025No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC95440.3041682588438738No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG86880.27688745105150625No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC86770.2765368799233333No Hit
GACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGACCCACTACCTGCATGTGAGCAGCAGTAATAAT86420.2754214263336921No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG81790.2606655688478671No Hit
ACCTGCATGTGAGCAGCAGTAATAATCAGCCTCGTCGTCAGCCTGGAGCC80020.25502456069453877No Hit
GTAATAATCAGCCTCGTCGTCAGCCTGGAGCCCAGAGATTTTCAGGGAGA79850.25448276895099875No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT72710.23172751572231834No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATTT70550.22484357356910412No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT70250.22388747049226881No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC68190.21732222936466633No Hit
GATTATTACTGCTGCTCACATGCAGGTAGTGGGTCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTGGGT66860.2130835057240298No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC66420.21168122121133798No Hit
CCCAAGACCCACTACCTGCATGTGAGCAGCAGTAATAATCAGCCTCGTCG64940.20696444603228378No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG64340.20505223987861315No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA63860.20352247495567666No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG63600.20269385228908607No Hit
ACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGACCCACTAC62670.1997299327508966No Hit
CTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGA61210.19507689777696474No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC59700.190264512290227No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC59640.19007329167485992No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC58780.18733246285459873No Hit
GGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC58060.18503781547019396No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA56720.18076722172699625No Hit
CTCTTAGTGTCCTCATAAATTATGAGTCGGGGGGCTTTGCCTGGATGTTG54690.1742975909070773No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT54080.1723535146508455No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG53190.16951707552290074No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT52860.1684653621383819No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC52860.1684653621383819No Hit
CTAAGAGGCCCTCAGGGATTTCTAATCGGTTCTCTGGTTCCAAGTCTGGC52410.16703120752312892No Hit
CATGCAGGTAGTGGGTCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGCCCC51940.1655333127027536No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT51820.16515087147201948No Hit
GTCGATCACCATTTCCTGCACTGGAACCAGCAATGATATTGGGAATTATG51430.16390793747213359No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC50950.1623781725491971No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA49200.15680090460099108No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG48890.1558129314215946No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC48850.1556854510113499No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGC47070.15001257275546037No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG46470.14810036660178977No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG46450.14803662639666743No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC46020.1466662119865368No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA45580.145263927473845No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC45060.1436066821406638No Hit
TTCCTGCACTGGAACCAGCAATGATATTGGGAATTATGACCTTGTCTCCTGGTACCAGCAACATCCAGGCAAAGC44860.14296928008944026No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC44300.14118455434601435No Hit
GCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATTTCCTGCA41380.13187848439815064No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA40740.1298387978342353No Hit
GCTTGGTCCCTCCGCCGAACACCCAAGACCCACTACCTGCATGTGAGCAGCAGTAATAATCAGCCTCGTCGTCAG40370.12865960403947177No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC39950.12732105973190233No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG39840.1269704886037294No Hit
CTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTC39200.12493080203981403No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC38600.12301859588614342No Hit
CGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGC38420.12244493404004224No Hit
GCAGTAATAATCAGCCTCGTCGTCAGCCTGGAGCCCAGAGATTTTCAGGG38410.12241306393748107No Hit
ACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACT38300.12206249280930812No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGACCCA38220.1218075319888187No Hit
TCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCC37770.12037337737356571No Hit
CCATTTCCTGCACTGGAACCAGCAATGATATTGGGAATTATGACCTTGTC37140.11836556091221156No Hit
GTACCAGCAACATCCAGGCAAAGCCCCCCGACTCATAATTTATGAGGACA36460.11619839393805154No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG36070.11495545993816562No Hit
GTTCTCTGGTTCCAAGTCTGGCAACACGGTCTCCCTGAAAATCTCTGGGC35230.11227837132302676No Hit
GCCTGGAGCCCAGAGATTTTCAGGGAGACCGTGTTGCCAGACTTGGAACC34980.11148161875899733No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA34460.10982437342581612No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC34150.10883640024641962No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG34130.10877266004129728No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA33910.10807151778495139No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT33480.10670110337482076No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC33370.10635053224664781No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA32840.10466141681090545No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG32560.10376905393919247No Hit
CCAGAGAACCGATTAGAAATCCCTGAGGGCCTCTTAGTGTCCTCATAAAT32420.103322872503336No Hit
GGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCC31860.10153814675991008No Hit
GTGTCTGGGTCTCCTGGACAGTCGATCACCATTTCCTGCACTGGAACCAG31810.1013787962471042No Hit
CTACCTGCATGTGAGCAGCAGTAATAATCAGCCTCGTCGTCAGCCTGGAG31700.10102822511893125No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT31660.10090074470868655No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)