FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758202

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758202
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3137737
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC195450.6229011545582055No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGACCCACTACCTGCATGTGAGCAG194830.6209252081994125No Hit
CTCCTGGACAGTCGATCACCATTTCCTGCACTGGAACCAGCAATGATATT143350.456857920214473No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATTTCCTG124330.3962409851431143No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT111340.3548417219161453No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG103410.3295687305851319No Hit
CACTAAGAGGCCCTCAGGGATTTCTAATCGGTTCTCTGGTTCCAAGTCTGGCAACACGGTCTCCCTGAAAATCT98510.31395238033015516No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC96300.30690908766413505No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG87640.27930957884615565No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG86170.27462467376966265No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTC82740.26369322859117894No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC79400.2530486143357458No Hit
GACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGACCCACTACCTGCATGTGAGCAGCAGTAATAAT76920.24514482890057387No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC76590.24409311551605506No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATTT74910.2387389382857773No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT72740.2318231260300019No Hit
GTAATAATCAGCCTCGTCGTCAGCCTGGAGCCCAGAGATTTTCAGGGAGA68440.21811898192869572No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC67020.2135934273650086No Hit
ACCTGCATGTGAGCAGCAGTAATAATCAGCCTCGTCGTCAGCCTGGAGCC66550.2120955325446333No Hit
CTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGA66440.21174496141646032No Hit
GATTATTACTGCTGCTCACATGCAGGTAGTGGGTCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTGGGT66130.21075698823706385No Hit
GGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC65530.20884478208339324No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT63590.2026619821865249No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA62630.1996024523406519No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC60630.19322843182841648No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG60320.19224045864901998No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG59580.18988207105949287No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG58360.18599391854702926No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC58030.18494220516251042No Hit
ACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGACCCACTAC57610.183603660854941No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT55840.17796265270161266No Hit
GTCGATCACCATTTCCTGCACTGGAACCAGCAATGATATTGGGAATTATG54590.1739788898814655No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC53920.17184359300986665No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG51730.1648640405489689No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA51020.16260126326712532No Hit
CTAAGAGGCCCTCAGGGATTTCTAATCGGTTCTCTGGTTCCAAGTCTGGC50520.16100775813906648No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT50170.15989230454942527No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA49570.15798009839575464No Hit
CTCTTAGTGTCCTCATAAATTATGAGTCGGGGGGCTTTGCCTGGATGTTG49040.15629098296001226No Hit
CTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTC48810.1555579706011052No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGC48750.15536674998573813No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT48470.15447438711402517No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC48260.15380511496024046No Hit
CATGCAGGTAGTGGGTCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGCCCC48220.15367763454999575No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG48210.15364576444743455No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC47180.1503631438836333No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA47030.14988509234521566No Hit
GCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATTTCCTGCA46690.14880150885813564No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC45730.14574197901226266No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC45700.14564636870457912No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG45470.14491335634567204No Hit
TTCCTGCACTGGAACCAGCAATGATATTGGGAATTATGACCTTGTCTCCTGGTACCAGCAACATCCAGGCAAAGC45380.14462652542262147No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG44740.14258683885870613No Hit
CCCAAGACCCACTACCTGCATGTGAGCAGCAGTAATAATCAGCCTCGTCG44500.14182195639723788No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC42500.13544793588500248No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC42060.1340456513723107No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC41260.1314960431674165No Hit
ACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACT40390.1287233442445941No Hit
GGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCT39400.12556820409103758No Hit
CGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGC38910.1240065690655399No Hit
GTTCTCTGGTTCCAAGTCTGGCAACACGGTCTCCCTGAAAATCTCTGGGC38460.12257241445028694No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT37420.11925792378392452No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG37380.11913044337367983No Hit
GCTTGGTCCCTCCGCCGAACACCCAAGACCCACTACCTGCATGTGAGCAGCAGTAATAATCAGCCTCGTCGTCAG36170.11527416096377739No Hit
TCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCC36160.11524229086121622No Hit
GTACCAGCAACATCCAGGCAAAGCCCCCCGACTCATAATTTATGAGGACA35940.11454114860487033No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGACCCA35590.11342569501522912No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA35150.11202341050253734No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC34600.11027055486167261No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC34430.1097287631181326No Hit
CCATTTCCTGCACTGGAACCAGCAATGATATTGGGAATTATGACCTTGTC34400.10963315281044905No Hit
GTGTCTGGGTCTCCTGGACAGTCGATCACCATTTCCTGCACTGGAACCAG33560.10695606419531017No Hit
GCAGTAATAATCAGCCTCGTCGTCAGCCTGGAGCCCAGAGATTTTCAGGG33350.10628679204152547No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC33330.10622305183640311No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAAC33180.10574500029798546No Hit
GCCTGGAGCCCAGAGATTTTCAGGGAGACCGTGTTGCCAGACTTGGAACC33100.10549003947749605No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT32760.10440645599041604No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA32710.10424710547761012No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG32030.1020799385034501No Hit
GGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG32010.10201619829832774No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA31990.1019524580932054No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC31610.10074139419588067No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA58600.041.7748031
GGTATCA23250.040.223331
TATCAAC66100.036.491992
ATCAACG69550.034.512693
TCAACGC70750.033.9726074
CAACGCA72950.032.9015
AACGCAG74300.032.4418456
ACGCAGA79950.030.1492087
CGCAGAG81000.029.8431668
AGAGTAC80650.029.80190311
CAGAGTA82400.029.29398310
ACTGGTG107250.028.50437770
GCAGAGT85400.028.3859929
CGAACTA854.539719E-628.32368729
AACGAAT907.167195E-626.70794517
GTGGTAT12700.024.9066981
AGTACGG56550.024.83367513
GAGTACT40000.024.72195612
AGTACTT40200.024.42813513
CCTGGTA86750.024.38465370
Produced by FastQC (version 0.11.5)