FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758203

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758203
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2327933
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCGTCAGGTCTCAGGCTGTTCATTTGTAGATACA94270.40495151707544846No Hit
GTCTATTACTGTGCGAGAGATTCGGGATTTAGTGGCTACGATTTTCTTGA65820.282740096042283No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT61490.2641399043701No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT56130.24111518673432614No Hit
GTATTACTGTCACCAGTATGGTAACTCACCTCGGACGTTCGGCCAAGGGA54300.23325413575047046No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT52070.2236748222564825No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT45760.19656923115914415No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT45120.19382001114293237No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG40660.17466138415495636No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT37020.1590251953127517No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA35140.1509493615151295No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGGGCTTGCTGGCCGTGGCACTCATTTA34990.15030501307382987No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGACCTCCAGCAACTTCGGCACCCAGAC33760.14502135585517278No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA33520.14399039834909338No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT32330.13887856738144955No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG31650.1359575211142245No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30810.1323491698429465No Hit
GCATGGAGGTGCATAATGCCAAGACAAAGCCACGGGAGGAGCAGTTCAACAGCACGTTCCGTGTGGTCAGCGTC30290.1301154285797744No Hit
GACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACAAGACAGTTGAGCGCAAATGTTGTGT29820.12809647013036887No Hit
GCCTGAGACCTGACGACACGGCTGTCTATTACTGTGCGAGAGATTCGGGA29190.1253902066769104No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29110.1250465541748839No Hit
GGGCTGGAGTGGGTGGCATTTATGCGCTATGATGGAAGCAATAAAAAATA29090.12496064104937728No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28830.12384377041779124No Hit
GTATGGTAACTCACCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT28260.12139524634085258No Hit
CCGCTGGTCAGAGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA28040.12045020196027978No Hit
CCTCCATGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGGCTCACGTCCACCACCACGCACGTGA27450.11791576475783452No Hit
CTCCTGCAGGGCCAGTCAGAGTGTAACCAGCAACTACTTAGCCTGGTACC27060.11624045881045546No Hit
CTCCTGTGCAGCCTCTGGATACCCCTTCAGTAGTTATGCAATCCACTGGGTCCGCCAGGCTCCAGGCAAGGGG26660.11452219630032308No Hit
GTGTATTACTGTCACCAGTATGGTAACTCACCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA26390.11336236910598373No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGGGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTAACCAG25390.10906671283065278No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGGATTGCATAACTAC25040.10756323313428694No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAACCAAAGGGCAG24890.1069188846929873No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC24870.10683297156748067No Hit
CACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTCCAACAA24500.10524357874560823No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24090.10348235967272254No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC23770.10210774966461662No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23640.10154931434882361No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16300.040.526751
TCTGGAG35800.040.40553370
GTATCAA40250.039.577031
TATCAAC44000.036.127352
GTCGTCA25800.034.1336816
CCTTTGT23350.033.91798870
TCAACGC46200.033.887144
TGTCGTC25950.033.8038115
ATCAACG46550.033.780183
TCGTCAG25950.033.67124617
CAACGCA46850.033.490435
TGACTAC19000.033.4730448
AACGCAG47700.033.038626
TCAGGTC26300.032.43974320
ACGATTT19750.032.2045738
CGTGTCG27100.031.86157613
CCGTGTC27200.031.49217212
GCCGTGT27250.031.181911
TACGATT20650.031.11965437
CTACTGG20600.031.09790651
Produced by FastQC (version 0.11.5)