FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005758204

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758204
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2327933
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCGTCAGGTCTCAGGCTGTTCATTTGTAGATACA85250.3662046974719633No Hit
GTCTATTACTGTGCGAGAGATTCGGGATTTAGTGGCTACGATTTTCTTGA68160.29279193172655743No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT55190.23707726983551503No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT54090.232352047932651No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT53620.23033308948324543No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT52220.22431917069778212No Hit
GTATTACTGTCACCAGTATGGTAACTCACCTCGGACGTTCGGCCAAGGGA47830.20546123964907925No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT39630.17023685819136547No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG36700.1576505853046458No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGACCTCCAGCAACTTCGGCACCCAGAC36620.15730693280261931No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT34440.14794240212239784No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA33260.14287352771750733No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT33010.14179961364867458No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA32520.13969474207376242No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGGGCTTGCTGGCCGTGGCACTCATTTA31630.1358716079887179No Hit
GTCCTGGTGCACGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGA30670.13174777796440018No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30580.1313611688996204No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT30470.13088864670933398No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29400.12629229449472987No Hit
GGGCTGGAGTGGGTGGCATTTATGCGCTATGATGGAAGCAATAAAAAATA28810.12375785729228461No Hit
CTCCTGTGCAGCCTCTGGATACCCCTTCAGTAGTTATGCAATCCACTGGGTCCGCCAGGCTCCAGGCAAGGGG28590.1228128129117118No Hit
GTCTATTACTGTGCGAGAGATTCGGGATTTAGTGGCTACGATTTTCTTGACTACTGGGGCCAGGGAACCCTGGT28070.1205790716485397No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27910.11989176664448677No Hit
GCCTGAGACCTGACGACACGGCTGTCTATTACTGTGCGAGAGATTCGGGA27390.11765802538131467No Hit
GCATGGAGGTGCATAATGCCAAGACAAAGCCACGGGAGGAGCAGTTCAACAGCACGTTCCGTGTGGTCAGCGTC27310.1173143728792882No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC27150.11662706787523523No Hit
GTATGGTAACTCACCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT26910.11559611036915582No Hit
GACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACAAGACAGTTGAGCGCAAATGTTGTGT26690.11465106598858302No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTT26670.11456515286307639No Hit
GTGTATTACTGTCACCAGTATGGTAACTCACCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA26660.11452219630032308No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26290.11293280347845062No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC25920.11134341065657817No Hit
CTCCTGCAGGGCCAGTCAGAGTGTAACCAGCAACTACTTAGCCTGGTACC25650.11018358346223882No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT25130.10794984219906671No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAACCAAAGGGCAG24670.1059738403124145No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24520.10532949187111486No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCACGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGAA24490.1052006221828549No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGGATTGCATAACTAC24410.10485696968082846No Hit
CCTCCATGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGGCTCACGTCCACCACCACGCACGTGA24270.1042555778022821No Hit
CCGCTGGTCAGAGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA24260.10421262123952879No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)